Decapeptide regulator of the HPG axis that stimulates GnRH, increasing LH/FSH and downstream sex steroids. It may enhance fertility, testosterone/estrogen production, sexual motivation, and support bone health.
Kisspeptin-10
Also known as: Metastin
Overview
Benefits
- Reproductive health
- Endocrine support
- Enhanced sexual drive
- Bone health
- Cancer prevention potential
- Kidney function support
Consider This Peptide If You Want To
- Support reproductive health and fertility via GnRH/LH/FSH
- Regulate hormones and improve libido
Dosage & Administration
Dosage Guidelines
Recommended Dosage
• Amount:100 mcg
• Frequency:weekdays
• Duration:4 weeks
• Rest Period:4 weeks
• Time of Day:morning
• Ingestion:subcutaneous
Administration Routes:Subcutaneous
Research Findings on Dosage:
• Subcutaneous Injection:
◦ Commonly Reported Dosage: 100-200 mcg or 1 mcg/kg up to 200-400 mcg daily, 3-5 days per week
◦ Duration: 1 month cycle with 1 month break
• Administration Notes:
◦ Administering in the morning in a fasted state mimics the body's natural testosterone production.
◦ May also be administered at bedtime
Mechanism of Action
Mechanism of Action
How this peptide works in the body
GnRH Secretion Stimulation:
Kisspeptin-10 binds to its receptor, GPR54 (KISS1R), a G-protein-coupled receptor located on hypothalamic neurons. This binding activates phospholipase C (PLC), which hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into two secondary messengers: inositol triphosphate (IP3) and diacylglycerol (DAG). This cascade ultimately triggers the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus, initiating the reproductive hormone cycle.
Calcium Mobilization:
The production of IP3 facilitates calcium release from the endoplasmic reticulum (ER) via IP3 receptors, increasing intracellular Ca²⁺ levels. This rise in calcium concentration plays a crucial role in depolarizing GnRH-secreting neurons and enhancing their excitability, further promoting pulsatile GnRH release into the hypophyseal portal system.
Protein Kinase Activation:
The simultaneous generation of DAG activates protein kinase C (PKC), which phosphorylates downstream targets, amplifying GnRH secretion. PKC signaling enhances the transcriptional activity of GnRH-encoding genes, reinforcing the sustained production and release of GnRH in response to kisspeptin-10 stimulation.
Hormonal Regulation:
Once released, GnRH binds to GnRH receptors (GnRHR) on gonadotrophs in the anterior pituitary, stimulating the synthesis and secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH)via the activation of the cyclic AMP (cAMP)/protein kinase A (PKA) and mitogen-activated protein kinase (MAPK)pathways. LH and FSH then act on the gonads, promoting testosterone production in Leydig cells and estrogen synthesis in ovarian granulosa cells, thus regulating spermatogenesis, ovulation, and overall reproductive function.
Consider Stacking With
- Any GHRH (CJC-1295, MOD-GRF-1295, Tesamorelin)
- Any GHRP (Ipamorelin, Hexarelin)
- 5-Amino-1MQ
- PT-141
- AOD-9604
- PEG-MGF
- Semaglutide/Tirzepatide
- IGF-1 LR3
- Melanotan-2
Side Effects & Cautions
Common Side Effects
- Injection site redness, itchiness, swelling
Research & References
Research Highlights
Fertility Support:
Clinical trials show increased LH and testosterone levels following kisspeptin-10 administration.
Improved ovulatory response in infertility treatments.
Behavioral Studies:
Enhanced limbic brain activity linked to sexual motivation.
Reduced mood disorders in reproductive health research.
Tumor Suppression:
Reduced metastasis in preclinical cancer models.
Correlation between low kisspeptin levels and cancer progression.
References
Comninos AN, Wall MB, Demetriou L, et al. "Kisspeptin modulates sexual and emotional brain processing in humans." Journal of Clinical Investigation, 2017
Dhillo WS, et al. "Effects of Kisspeptin-10 on Gonadotropin Levels in Healthy Male and Female Volunteers." Journal of Clinical Endocrinology & Metabolism, 2023
Mead EJ, Maguire JJ, Kuc RE, Davenport AP. "Kisspeptins: Multifunctional Peptides in Reproduction, Cancer, and Cardiovascular Health." British Journal of Pharmacology, 2007
Bhattacharya M, Babwah AV. "Kisspeptin: Beyond the Brain." Endocrinology, 2015
Harter CJL, Kavanagh GS, Smith JT. "The Role of Kisspeptin Neurons in Reproduction and Metabolism." Journal of Endocrinology, 2018