SNAP-8

SNAP-8 is a topical octapeptide that mimics part of SNAP-25 to inhibit neurotransmitter release and reduce facial muscle contractions, softening wrinkles and expression lines as a non-invasive alternative to Botox.

- Reduce fine lines and expression wrinkles

- Use non-invasively for skin rejuvenation

Mechanism of Action

How this peptide works in the body

SNARE Complex Disruption: SNAP-8 is a synthetic peptide designed to mimic the N-terminal end of the SNAP-25 protein, a core component of the SNARE complex involved in synaptic vesicle fusion. Normally, the SNARE complex—composed of SNAP-25, syntaxin, and synaptobrevin (VAMP)—mediates the calcium-dependent release of acetylcholine at the neuromuscular junction. By competitively binding in place of SNAP-25, SNAP-8 destabilizes this complex and inhibits vesicle docking and neurotransmitter exocytosis. Inhibition of Acetylcholine Release: Acetylcholine is the principal neurotransmitter responsible for signaling muscle contractions in facial muscles. By disrupting SNARE complex formation, SNAP-8 reduces acetylcholine release into the synaptic cleft. This lowers muscle fiber excitation and dampens repetitive micro-contractions associated with facial expressions, particularly in dynamic areas like the forehead and periocular region. Reduced Muscle Fiber Hyperactivity: The decreased availability of acetylcholine leads to a downregulation of post-synaptic muscle receptor activation, specifically nicotinic acetylcholine receptors (nAChRs). As these receptors are stimulated less frequently, sustained contraction cycles are minimized. Over time, this results in visible smoothing of superficial expression lines and reduced wrinkle depth. Modulation of Calcium-Mediated Vesicle Fusion: The SNARE complex works downstream of voltage-gated calcium channel activation. When SNAP-8 interferes with SNARE function, it indirectly reduces the calcium-triggered vesicle fusion events needed for neurotransmitter release. This diminishes the intracellular response to depolarization in presynaptic neurons, contributing to long-term muscle relaxation in the treated regions. Impact on Dermal Homeostasis: By attenuating repetitive contraction-induced mechanical stress in the dermis, SNAP-8 reduces mechanotransduction pathways that would otherwise activate matrix metalloproteinases (MMPs) such as MMP-1 and MMP-9. These enzymes are responsible for breaking down collagen and elastin. As MMP activity declines, the dermal matrix is better preserved, supporting skin elasticity and preventing structural aging. Neuropeptide Signaling Modulation: Emerging research suggests SNAP-8 may also influence local neuropeptide release, including substance P and calcitonin gene-related peptide (CGRP). These molecules contribute to local inflammation and vasodilation in the skin. By moderating their release, SNAP-8 may offer additional anti-inflammatory and skin-calming benefits.